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KMID : 0984920150170020018
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Journal of Skin Barrier Research
2015 Volume.17 No. 2 p.18 ~ p.118
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Activation of Toll Like Receptor 1 and 6 by House Dust Mite Enhances the Expression of Tight Junction Protein in Epidermal Keratinocytes
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Jang Yong-Hyun
Moon Sun-Young
Sim Hyun-Bo
Kim Sang-Lim
Lee Weon-Ju
Lee Seok-Jong
Choi Jin-Kyeong
Kim Sang-Hyun
Kim Do-Won
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Abstract
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The function of innate receptors has recently been extended to include epithelial barrier regulation. Toll like receptor(TLR)2 activation was shown to aid tight junction (TJ) repair in atopic dermatitis. However, the role of other TLRs in modulating epidermal barrier in vitro and in vivo remains poorly understood. We investigated the changes of TJ proteins following the activation of TLR 1 and 6 by house dust mite (HDM). Firstly, the mRNA levels of toll like receptors and TJ elements were assayed using real-time RCR in epidermal keratinocytes and a murine model with AD-like lesions activated by HDM allergen, Dermatophagoides farinae extracts (DFE). Additionally, the association between specific TLR activation and the changes of certain TJ proteins was studied in TLR deficient mice. Both in vitro and in vivo, DFE activated TLR 1 and TLR 6. In addition, DFE upregulated gene expression of TJ proteins including claudin-1, claudin-23 and occludin. These results provide the evidence that HDM triggers the activation of PRRs such as TLR 1 and TLR 6 and thus stimulates the modulation of TJ proteins. Moreover, gene expression of claudin-1, claudin-23 and occludin was not increased in TLR1 (tlr1(-/-)) and TLR6 (tlr6(-/-)) knockout mice. Our data suggest that the innate immune activation of the keratinocytes by HDM allergens contribute to lead the preservation of TJ integrity
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KEYWORD
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House dust mite, Keratinocyte, Tight junction, Toll like receptor
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